Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®, Carrier-free

• Background:

  TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

• :

  heparin or EDTA

• Description:

  Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	57	57	66	64	61	4.06	6.6%
  Sample 2	238	272	240	245	248	13.66	5.5%
  Sample 3	848	982	998	845	918	71.98	7.8%

  *number of samples for each test n=16.

• Synonyms:

  Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;TFPI;LACI, TFPI1;

• Gene Name:

  Tissue factor pathway inhibitor

• Gene ID:

  7035

• UniProt:

  P10646

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D29-K282

• Tissue Specificity:

  Mostly in endothelial cells.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Inhibits factor X (X (a)) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  35015 MW

• Specificity:

  Natural and recombinant human TFPI

• Subcellular Location:

  Isoform Alpha: Secreted.

• Sequence Similarities:

  Contains 3 BPTI/Kunitz inhibitor domains.

• Protein Name:

  Tissue factor pathway inhibitor