Human ICAM-1/CD54 PicoKine® Quick ELISA Kit, Carrier-free

Human ICAM-1/CD54 PicoKine® Quick ELISA Kit, Carrier-free

• Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
• Description: Human ICAM-1/CD54 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Gene Name: intercellular adhesion molecule 1
• Gene ID: 3383
• UniProt: P05362
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q28-E480
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Specificity: Natural and recombinant Human ICAM1
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	280	265	279	257	270	5.68	2.1%
  Sample 2	2022	1702	1836	1759	1830	84.17	4.6%
  Sample 3	4863	4823	4469	4578	4683	159.23	3.4%

  *number of samples for each test n=16.
• : heparin or EDTA
• Protein Name: Intercellular adhesion molecule 1