Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody, Carrier-free

• Description:

  Boster Bio Anti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody catalog # P01906. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

• Phospho Site:

  S293

• Synonyms:

  Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;3.1.3.16;Replication protein C;RP-C;PPP2CA;

• Gene Name:

  Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

• UniProt:

  P08559

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  A synthesized peptide derived from human Phospho-PDHA1 (S293)

• Clonality:

  Monoclonal

• Clone:

  Clone: 25P34

• Tissue Specificity:

  Expressed in brain, and to a lesser extent in lung, kidney and testis.

• Applications:

  WB,IHC,IP

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Function:

  PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. .

• Components:

  Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

• Storage Conditions:

  Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  35594 MW

• Observed Molecular Weight:

  43 kDa

• Fragment:

  IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  IP 1:50

• Subcellular Location:

  Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). .