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TPPP/p25 Antibody

CAT: 0013-GTR17698228Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17698228Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to TPPP
Product Name Alternative
Anti brain specific protein p25 alpha antibody, anti glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody, anti p24 antibody, anti p25 antibody, anti p25alpha antibody, anti TPPP/p25 antibody, anti TPPP1 antibody, anti tubulin polymerization promoting protein antibody, anti tubulin polymerization-promoting protein antibody, anti TPPP antibody
Reactivity
Human, Mouse, Rat
Target
TPPP/p25
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DPSKDRAAKRLSLES
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.001-0.01 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
23.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313 (20) :4091-106; PMID: 18028908) . Recommended concentration: 0.001-0.01μg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2μg/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75μg/ml.
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
11076

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