Anti-NEI3 Antibody

• Description:

  Rabbit Polyclonal Antibody specific to NE13

• Product Name Alternative:

  EC 3.2.2.-, EC 4.2.99.18, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3

• Gene Name:

  NEIL3

• NCBI Gene ID:

  NEIL3

• UniProt:

  Q8TAT5

• Accession Number:

  NP_060718

• Cellular Locus:

  Nucleus, Chromosome

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide corresponding to amino acids 164-177 (LRAESEVKKQKGRMLG) of human NEI3 conjugated to KLH at the C- terminus.

• Target Antigen:

  Endonuclease 8-like 3

• Target:

  NEI3

• Clonality:

  Polyclonal

• Type:

  Antibody

• Applications:

  WB

• Field of Research:

  DNA

• Purification Method:

  Purified by peptide immuno-affinity chromatography

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium which is identical to that used in the assay system.

• Format:

  Purified

• Form:

  Liquid

• Buffer:

  Phosphate Buffered Saline

• Function:

  DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also rUniProtKB:A0A1L8HU22, PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964}.

• Additionnal Information:

  Immunoblotting: use at 1:500 dilution. A band of approximately 68 kDa is detected.
  
  Positive control: human testes tissue lysate.

• Storage Conditions:

  This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

• Specificity:

  This antibody recognizes human NEI3 protein. Sequence homology to mouse and rat is 85%.

• Formulation:

  PBS, pH 7.4.

• Buffer pH:

  pH 7.4

• Target Background:

  Prokaryotes and lower eukaryotes possess redundant activities that remove the oxidative DNA base damages produced during normal oxidative metabolism and which have been associated with cancer and aging. Thus far, only one oxidized pyrimidine- specific DNA glycosylase has been identified in humans, hNthl. NEI3 is one of three new putative human DNA glycosylases.

• CAS Number:

  9007-83-4