Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®, APC Conjugate

• Background:

  Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

• Description:

  Boster Bio Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody catalog # PA1410. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha;PIK3R1;GRB1;

• Gene Name:

  Phosphatidylinositol 3-kinase regulatory subunit alpha

• UniProt:

  P27986

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha, identical to the related rat and mouse sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). .

• Applications:

  WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). .

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Volinia S, Patracchini P, Otsu M, Hiles I, Gout I, Calzolari E, Bernardi F, Rooke L, Waterfield MD (May 1992). "Chromosomal localization of human p85 alpha, a subunit of phosphatidylinositol 3-kinase, and its homologue p85 beta". Oncogene 7 (4): 789–93. PMID 1314371. 2. Cannizzaro, L. A., Skolnik, E. Y., Margolis, B., Croce, C. M., Schlesinger, J., Huebner, K.The human gene encoding phosphatidylinositol 3-kinase associated p85-alpha is at chromosome region 5q12-13.Cancer Res.51: 3818-3820, 1991.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  83598 MW

• Observed Molecular Weight:

  85 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
  

• Sequence Similarities:

  Belongs to the PI3K p85 subunit family.

• Protein Name:

  Phosphatidylinositol 3-kinase regulatory subunit alpha(PI3-kinase regulatory subunit alpha/PI3K regulatory subunit alpha/PtdIns-3-kinase regulatory subunit alpha)