Anti-EPLIN/LIMA1 Antibody Picoband®, FITC Conjugate

• Background:

  LIM domain and actin-binding protein 1 is a protein that in humans is encoded by the LIMA1 gene. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.

• Description:

  Boster Bio Anti-EPLIN/LIMA1 Antibody Picoband® catalog # A05231-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Zinc finger protein Helios; Ikaros family zinc finger protein 2; IKZF2; HELIOS, ZNFN1A2

• Gene Name:

  IKAROS family zinc finger 2

• Gene ID:

  51474

• UniProt:

  Q9UHB6

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human EPLIN/LIMA1 recombinant protein (Position: R9-E744).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitous. High expression in liver, spleen, pancreas and kidney.

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Associates with Ikaros at centromeric heterochromatin.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Chen, S., Maul, R. S., Kim, H. R., Chang, D. D. Characterization of the human EPLIN (Epithelial Protein Lost in Neoplasm) gene reveals distinct promoters for the two EPLIN isoforms. Gene 248: 69-76, 2000. 2. Maul, R. S., Chang, D. D. EPLIN, epithelial protein lost in neoplasm. Oncogene 18: 7838-7841, 1999. 3. Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D., Chang, D. D. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160: 399-407, 2003.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  39411 MW

• Observed Molecular Weight:

  85 kDa, 100 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus.

• Protein Name:

  Zinc finger protein Helios