Anti-Ki67 Antibody (monoclonal, 5E12)-FITC Conjugate

• Background:

  Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

• Description:

  Boster Bio Anti-Ki67 Antibody Picoband® (monoclonal, 5E12) catalog # M00254-8. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

• Synonyms:

  Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

• Gene Name:

  tuberous sclerosis 2

• Gene ID:

  4288

• UniProt:

  P46013

• Host:

  Mouse

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

• Clonality:

  Monoclonal

• Clone:

  Clone: 5E12

• Tissue Specificity:

  Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

• Applications:

  IHC,ICC,IF,Flow Cytometry

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a activator of the protein kinase activity of mTORC1 (PubMed:15340059). May also play a role in microtubule-mediated protein transport (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• References & Citations:

  1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. 2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989. 3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  35-60 kDa

• Fragment:

  Mouse IgG2b

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  

• Subcellular Location:

  Cytoplasm; Membrane; Peripheral membrane protein

• Protein Name:

  Glial fibrillary acidic protein