Anti-Ki67 Antibody (monoclonal, 5E12)

• Background:

  Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

• Description:

  Boster Bio Anti-Ki67 Antibody Picoband® (monoclonal, 5E12) catalog # M00254-8. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

• Synonyms:

  Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

• Gene Name:

  Tuberous sclerosis 2

• UniProt:

  P46013

• Host:

  Mouse

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256) .

• Clonality:

  Monoclonal

• Clone:

  Clone: 5E12

• Tissue Specificity:

  Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

• Applications:

  IHC, ICC, IF, Flow Cytometry

• Field of Research:

  Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways and Processes, Signal Transduction, Signaling Pathway

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400) . Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a activator of the protein kinase activity of mTORC1 (PubMed:15340059) . May also play a role in microtubule-mediated protein transport (By similarity) . Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity) .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Mouse IgG2b

• Applications Notes:

  6

• Other Gene Names:

  MKI67

• Subcellular Location:

  Cytoplasm; Membrane; Peripheral membrane protein

• Protein Name:

  Glial fibrillary acidic protein