Anti-Hexokinase 1/HK1 Antibody Picoband®, FITC Conjugate

• Background:

  Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

• Description:

  Boster Bio Anti-Hexokinase 1/HK1 Antibody Picoband® catalog # A01504-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I; HK1

• Gene Name:

  hexokinase 1

• Gene ID:

  3098

• UniProt:

  P19367

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.

• Applications:

  ELISA,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  3'-exoribonuclease that has a preference for poly (A) tails of mRNAs, thereby efficiently degrading poly (A) tails. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly (A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly (A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly (A) tails of AREs mRNAs, which constitutes the first step of destabilization.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

• References & Citations:

  1. Andreoni, F., Ruzzo, A., Magnani, M. Structure of the 5-prime region of the human hexokinase type I (HKI) gene and identification of an additional testis-specific HKI mRNA. Biochim. Biophys. Acta 1493: 19-26, 2000. 2. Bianchi, M., Crinelli, R., Serafini, G. Giammarini, C., Magnani, M. Molecular bases of hexokinase deficiency. Biochim. Biophys. Acta 1360: 211-221, 1997. 3. Bianchi, M., Magnani, M. Hexokinase mutations that produce nonspherocytic hemolytic anemia. Blood Cells Mol. Dis. 21: 2-8, 1995.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  102 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Mitochondrion outer membrane.