Products for Research Use Only

Recombinant Mouse Leukemia inhibitory factor (Lif) (Active)

CAT: 0013-GTR19063865Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19063865Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Mouse Leukemia inhibitory factor (Lif) (Active) spans the amino acid sequence from region 24-203aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Name Alternative
Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
UniProt
P09056
Expression Region
24-203aa
Tag
Tag free
Source
Mammalian cell
Origin
Mus musculus (Mouse)
Field of Research
Cancer Biology; Immunology & Inflammation
Endotoxin
≤10 EU/mg by the LAL method
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
Measured in the M1 cell differentiation assay. The ED50 is ≤0.2ng/ml.
Form
Lyophilized powder
Molecular Weight
19.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm filtered solution containing PBS,5%mannitoland0.01% Tween 80, pH 7.4
AA Sequence
SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF

UniProtKB · P09056

Leukemia inhibitory factor

LIF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P09056
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lif
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
LIF
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal