Anti-Interferon regulatory factor 8 IRF8 Antibody Picoband®

• Background:

  Interferon regulatory factor 8 (IRF8) also known as Interferon consensus sequence-binding protein (ICSBP), is a protein that in humans is encoded by the IRF8 gene. By genomic sequence analysis, the IRF8 gene is mapped to chromosome 16q24.1. Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 is a transcription factor that plays critical roles in the regulation of lineage commitment and in myeloid cell maturation including the decision for a common myeloid progenitor (CMP) to differentiate into a monocyte precursor cell.

• Description:

  Boster Bio Anti-Interferon regulatory factor 8 IRF8 Antibody catalog # PA2209. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Interferon regulatory factor 8; IRF-8; Interferon consensus sequence-binding protein; H-ICSBP; ICSBP; IRF8; ICSBP1

• Gene Name:

  Interferon regulatory factor 8

• UniProt:

  Q02556

• Host:

  Rabbit

• Reactivity:

  Rat, Mouse, Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human IRF8, different from the related mouse and rat sequences by two amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Predominantly expressed in lymphoid tissues. .

• Applications:

  WB

• Field of Research:

  Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, TLR Signaling, Transcription

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS) ) . Plays a negative regulatory role in cells of the immune system. Involved in CD8 (+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity) . .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
  

• Other Gene Names:

  IRF8

• Subcellular Location:

  Nucleus .

• Sequence Similarities:

  Belongs to the IRF family.

• Protein Name:

  Interferon regulatory factor 8