Anti-TRPM4 Antibody Picoband®

• Background:

  Transient receptor potential cation channel subfamily M member 4 (hTRPM4), also known as melastatin-4, is a protein that in humans is encoded by the TRPM4 gene. It is mapped to 19q13.33. The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-TRPM4 Antibody Picoband® catalog # PB9902. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Transient receptor potential cation channel subfamily M member 4; hTRPM4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; LTrpC-4; LTrpC4; Melastatin-4; TRPM4; LTRPC4

• Gene Name:

  Transient receptor potential cation channel subfamily M member 4

• UniProt:

  Q8TD43

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human TRPM4 recombinant protein (Position: F1079-D1214) . Human TRPM4 shares 76.8% and 77.8% amino acid (aa) sequence identity with mouse and rat TRPM4, respectively.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells. .

• Applications:

  IHC, WB

• Field of Research:

  Cancer, Metabolism, Neuroscience, Neurotransmission, Plasma Membrane, Receptors / Channels, Signal Transduction

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca (2+), it is impermeable to it. Mediates transport of monovalent cations (Na (+) > K (+) > Cs (+) > Li (+) ), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca (2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca (2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
  

• Other Gene Names:

  TRPM4

• Subcellular Location:

  Isoform 1: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus.

• Protein Name:

  Transient receptor potential cation channel subfamily M member 4