TLR4/IL-8 Luciferase Reporter-HeLa Cell Line

• Description:

  The TLR4/IL-8 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses human TLR4, MD-2 and CD14 as well Renilla luciferase reporter gene under the transcriptional control of the IL-8 promoter. IL-8 is one of the major pro-inflammatory cytokines induced by ligand (such as LPS) -mediated Toll-like receptor 4 (TLR4) activation. TLR4 is one of the key innate immune receptors, which is activated by LPS and can lead to sepsis upon dysregulation. The TLR4/IL-8 activation by LPS is shown in Figure 1.

• Gene Name:

  TLR4/IL-8

• Applications:

  Functional Assay

• Dilution:

  Application: Culture conditions: Functional validation:

• Shipping Conditions:

  Available, requires dry ice shipping

• Storage Conditions:

  Immediately upon receipt, store in liquid nitrogen.

• Applications Notes:

  Functional Assay, detecting the transcriptional activity of TLR4/IL-8