Anti-HIF-alpha HIF1A Monoclonal Antibody

• Description:

  Boster Bio Anti-HIF-alpha HIF1A Monoclonal Antibody catalog # M00013-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.

• Synonyms:

  Hypoxia-inducible factor 1-alpha

• Gene Name:

  HIF1A

• UniProt:

  Q16665

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Monkey

• Immunogen:

  Purified recombinant fragment of human HIF-1α expressed in E. Coli.

• Clonality:

  Monoclonal

• Clone:

  Clone: 1A3

• Tissue Specificity:

  Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

• Applications:

  WB, IHC, IF, ELISA

• Purification:

  Affinity purification

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity) . Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

• Molecular Weight:

  92670 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG

• Other Gene Names:

  Hypoxia-inducible factor 1-alpha

• Subcellular Location:

  Cytoplasm

• Protein Name:

  Hypoxia-inducible factor 1-alpha