Anti-STAT1 Purified Monoclonal Antibody

• Background:

  STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

• Description:

  Boster Bio Anti-STAT1 Purified Monoclonal Antibody (Catalog# M00036-3) . Tested in IP, WB, IHC-P application (s) . This antibody reacts with Mouse, Human.

• Synonyms:

  CANDF7, IMD31, ISGF3

• Gene Name:

  STAT1

• UniProt:

  P42224

• Host:

  Mouse

• Reactivity:

  Mouse, Human

• Immunogen:

  STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE) . The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

• Clonality:

  Monoclonal

• Clone:

  Clone: SM1

• Applications:

  IP, WB, IHC-P

• Purification:

  Purified by protein-A affinity chromatography.

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426) . ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788) . It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

• Molecular Weight:

  87335 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Fragment:

  Mouse IgG2b

• Other Gene Names:

  Signal transducer and activator of transcription 1

• Subcellular Location:

  Cytoplasm

• Protein Name:

  Signal transducer and activator of transcription 1-alpha/beta