Anti-Histone H3 (mono methyl K9) Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-Histone H3 (mono methyl K9) Rabbit Monoclonal Antibody catalog # M12477-11. Tested in WB, IHC, ICC/IF, ChIP applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ

• Gene Name:

  Histone H3.1

• UniProt:

  P68431

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from Histone H3 (mono methyl K9)

• Clonality:

  Monoclonal

• Clone:

  Clone: 26H43

• Tissue Specificity:

  Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

• Applications:

  WB, IHC, ICC, IF, ChIP

• Field of Research:

  2339, Epigenetics and Nuclear Signaling, Histones, Unmodified

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  ChIP 1:50

• Other Gene Names:

  H3C1

• Subcellular Location:

  Nucleus. Chromosome.

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain