Mouse CCL12/MCP5 PicoKine® Quick ELISA Kit

• Background:

  Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

• :

  heparin or EDTA

• Reproducibility:

  Reproducibility

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	86	90	78	82	84	4.28	5.1%
  Sample 2	251	284	278	261	268	13.13	4.9%
  Sample 3	901	899	850	891	885	45.13	5.1%

  *number of samples for each test n=16.

• Description:

  Mouse CCL12/MCP5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CCL12/MCP51 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• CAS Number:

  7732-18-5

• Gene Name:

  Ccl12

• Gene ID:

  20293

• UniProt:

  Q62401

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: G23-G104

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Components:

  Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

• Storage Conditions:

  Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

• Specificity:

  Natural and recombinant Mouse Ccl12