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Fluo-4 Calcium Assay Kit

CAT: 0749-KTA7010-01Size: 200 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTA7010-01Size:200 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell.
Tag
Fluo-4
Type
Cell analysis
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stored at -20°C for 12 months, protected from light
Applications Notes
Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration.
Other Product Names
Fluo-4
Recommended Usage
•If a medium containing serum is used, the esterase in the serum will hydrolyze Fluo-4 AM, thus reducing the effectiveness of Fluo-4 AM in entering cells. In addition, the medium containing phenol red will make the background value slightly higher, and the residual medium should be removed as far as possible before adding the Stain Solution. • Ionomycin is an ionic vector with affinity for calcium. The increase in green fluorescence intensity can be observed within a few seconds after Ionomycin treatment. It is recommended to use laser confocal microscope to continuously take photos to detect the change of intracellular calcium concentration.

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