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Caspase-3 Assay Kit (Colorimetric)

CAT: 0749-KTA3022-01Size: 20 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTA3022-01Size:20 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12) . Caspase-3, also known as Yama, CPP32, and apopain, cleaves its substrates at the carboxyl terminus of aspartate residues. Active Caspase-3 consists of 2 sets of homodimers (17 and 12 kDa) that are derived from two precursor Caspase-3 polypeptides and has two active sites. Caspase-3, together with Caspases 8 and 9, is situated at pivotal junctions in apoptotic pathways.
Description
Caspase-3 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-3.
Tag
Caspase
Type
Cell analysis
Assay Type
Colorimetric
Components
• Cell Lysis Buffer • Reaction Buffer (2X) • Ac-DEVD-pNA (4mM) • pNA (10mM) • DTT (100X)
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Simple and optimized procedure. • Fast and convenient. • Detect early/middle stages of apoptosis.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Applications Notes
Caspase 3 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroanilide) by caspase 3, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.

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