Mouse Sonic Hedgehog Protein

CAT: 0749-PRP1132-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0749-PRP1132-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mouse Sonic Hedgehog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH) . Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region.The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N terminal fragment (SHHN), and a catalyticprocessing, glycosylated, 34 kDa, 239 aa C terminal fragment.
Description
Mouse Sonic Hedgehog Protein, expressed in E. coli
Product Name Alternative
HHG1 Protein; HLP3 Protein; HPE3 Protein; MCOPCB5 Protein; SMMCI Protein; Sonic hedgehog Protein; TPT Protein; TPTPS Protein
Gene ID
20423
Accession Number
Q62226
Tag
SHH
Type
Cytokines and proteins
Applications
No tag
Purity
> 96 % as determined by SDS-PAGE
Activity
Testing in progress
Molecular Weight
19.8 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin : < 0.1 EU per 1 μg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Mouse SHH protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Mouse SHH consists of 174 amino acids and has a predicted molecular mass of 19.8 kDa
Formulation
Lyophilized from sterile PBS, pH 7.4.
Applications Notes
Mouse
Other Product Names
HHG1 Protein; HLP3 Protein; HPE3 Protein; MCOPCB5 Protein; SMMCI Protein; Sonic hedgehog Protein; TPT Protein; TPTPS Protein
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse SHH protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/Q62226
AA Sequence
Amino acid sequence derived from Mouse SHH (Q62226) (Cys25-Gly198) was expressed.

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