Human Sonic Hedgehog Protein

Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH) . Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important roles in the division of adult stem cells and the development of certain cancers and other diseases.SHH, DHH and IHH are structurally related, and share a high degree of amino acid sequence identity (e.g. SHH and IHH are 93% identity) . The biologically active form of each HH molecule is obtained by autocatalytic cleavage of their precursor proteins, and each corresponds to approximately one half of the N-terminal portion of the precursor molecule. Although HH proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can be substituted for one another in experimental systems. The recombinant E. coli-derived Human Sonic Hedgehog including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified, Cys residue

Human Sonic Hedgehog Protein, expressed in E. coli

HHG1 Protein, Human; HLP3 Protein, Human; HPE3 Protein, Human; MCOPCB5 Protein, Human; SMMCI Protein, Human; Sonic hedgehog Protein, Human; TPT Protein, Human; TPTPS Protein, Human

6469

Q15465

SHH

Cytokines and proteins

No tag

> 98 % as determined by SDS-PAGE

Testing in progress

23.84 kDa

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Endotoxin: < 1 EU per μg of the protein as determined by the LAL method

Gel pack with blue ice.

Lyophilized recombinant Human SHH protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

The recombinant Human SHH consists of 176 amino acids with 6xHis tag at N terminus and predicts a molecular mass of 23.84 kDa

Lyophilized from PBS, pH 7.5.

Human

HHG1 Protein; HLP3 Protein; HPE3 Protein; MCOPCB5 Protein; SMMCI Protein; Sonic hedgehog Protein; TPT Protein; TPTPS Protein

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human SHH protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

E. coli

https://www.uniprot.org/uniprot/Q15465