Recombinant Rat Stem Cell Factor

• Background :

  Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75 % ­ 90 % a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.

• Product Name Alternative :

  Hematopoietic growth factor KL、MGF

• Gene ID :

  60427

• UniProt :

  P21581

• Target :

  MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

• Purification :

  > 95 % by SDS-PAGE and HPLC analyses.

• Endotoxin :

  Less than 1 EU/ug of rRtSCF as determined by LAL method.

• Bioactivity :

  5

• Storage Conditions :

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

• Calculated Molecular Weight :

  Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.

• Formulation :

  Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

• Host or Source :

  Escherichia coli