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Recombinant Canine Stem Cell Factor

CAT: 0355-AP60219Size: InquireDry Ice: NoHazardous: No
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CAT#:0355-AP60219Size:Inquire
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis and melanogenesis and it promotes mast cell adhesion, migration, proliferation, and survival. Soluble canine SCF shares 88 %, 93 %, 86 %, 83 %, 76 %, 76 %, 86 % and 88 % a.a. sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes .
Product Name Alternative
Hematopoietic Growth Factor KL, MGF
Gene ID
403507
UniProt
Q06220
Target
KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA
Purification
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 1 EU/ug of rCaSCF as determined by LAL method.
Bioactivity
5
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.
Host or Source
Escherichia coli

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