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Recombinant Human tPA beta chain

CAT: 0013-GTR19035373-01Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19035373-01Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human tPA beta chain
Product Name Alternative
Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA, t-Plasminogen activator
UniProt
P00750
Expression Region
311-562
Reactivity
Human
Tag
C-terminal hFc-His Tag
Source
HEK293 Cells
Purity
Greater than 95% as determined by SDS-PAGE
Molecular Weight
60-65 kDa
Storage Conditions
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Notes
For research use only.
Applications Notes
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Tested Applications
ELISA, WB
Preservative
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4

UniProtKB · P00750

Tissue-type plasminogen activator

TPA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00750
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PLAT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
t-PA, t-plasminogen activator, tPA
EC number
3.4.21.68
Processing
Precursor
Secondary accessions
A8K022, B2R8E8, Q15103, Q503B0, Q6PJA5, Q7Z7N2, Q86YK8, Q9BU99, Q9BZW1
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinZymogen

Domain

EGF-like domainKringleRepeatSignal

Molecular function

HydrolaseProteaseSerine protease

Biological process

Plasminogen activation

Cellular component

Secreted

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