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Recombinant Human Sonic Hedgehog N-Terminus

CAT: 0013-GTR19033556-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19033556-01Size:5 µg
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Description
Recombinant Human Sonic Hedgehog N-Terminus
Product Name Alternative
HHG-1
UniProt
Q15465
Source
Escherichia coli
Endotoxin
Less than 1 EU/ug of rHuSHH as determined by LAL method.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x103 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Molecular Weight
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

UniProtKB · Q15465

Sonic hedgehog protein

SHH_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15465
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SHH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SHH
EC number
3.1.-.-
Processing
Precursor
Secondary accessions
A4D247, Q75MC9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Autocatalytic cleavageGlycoproteinLipoproteinPalmitate

Ligand

CalciumMetal-bindingZinc

Cellular component

Cell membraneEndoplasmic reticulumGolgi apparatusMembraneSecreted

Molecular function

Developmental proteinHydrolaseProteaseTransferase

Disease

Disease variantHoloprosencephalyMicrophthalmia

Domain

Signal

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