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Turbo Nuclease

CAT: 0013-GTR19031025Size: 10000 UnitsDry Ice: NoHazardous: No
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CAT#:0013-GTR19031025Size:10000 Units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single-or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner. Endonuclease from Serratia macescens recombinant expressed and purified from E.coli. Catalytic activity from pH 6 to 10 (optimal around 8.8) and tem-perature 0 to 44 °C. High catalytic efficiency (34-fold greater than DNase I) . Activity requires the presence of Mg2+ (optimum 2 mM) .
Product Name Alternative
Also known as Benzonase, Serratia marcescens, recombinant, E. coli
Source
E. coli
Origin
Serratia marcescens
Concentration
250 units/μl
Form
Liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl 2, 1 mM DTT and 50% [v/v] glycerol)
Storage Conditions
Store at -20 °C. avoid freeze/thaw cycles
Notes
For research use only.
Applications Notes
Turbo Nuclease is very effective in degrading nucleic acid from pro-tein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required: Reduction of viscosity from cell lysates. Prevention of cell clumping. Elimination of unspecific protein-DNA complexes prior 2D-or native gel electrophoresis. Removal of nucleic acids from large-scale protein preparations.

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