Phospho-Beta Catenin (S552) Recombinant Rabbit Monoclonal Antibody [PSH08-72]

Phospho-Beta Catenin (S552) Recombinant Rabbit Monoclonal Antibody [PSH08-72]

• Description: The cellular level of β-catenin is mostly controlled by its ubiquitination and proteosomal degradation. The E3 ubiquitin ligase TrCP1 (also known as β-TrCP) can recognize β-catenin as its substrate through a short linear motif on the disordered N-terminus. However, this motif (Asp-Ser-Gly-Ile-His-Ser) of β-catenin needs to be phosphorylated on the two serines in order to be capable to bind β-TrCP. Phosphorylation of the motif is performed by Glycogen Synthase Kinase 3 alpha and beta (GSK3α and GSK3β). GSK3s are constitutively active enzymes implicated in several important regulatory processes. There is one requirement, though: substrates of GSK3 need to be pre-phosphorylated four amino acids downstream (C-terminally) of the actual target site. Thus it also requires a "priming kinase" for its activities. In the case of β-catenin, the most important priming kinase is Casein Kinase I (CKI). Once a serine-threonine rich substrate has been "primed", GSK3 can "walk" across it from C-terminal to N-terminal direction, phosphorylating every 4th serine or threonine residues in a row. This process will result in dual phosphorylation of the aforementioned β-TrCP recognition motif as well.
• Product Name Alternative: b-catenin antibody Beta catenin antibody Beta-catenin antibody Cadherin associated protein antibody Catenin (cadherin associated protein), beta 1, 88kDa antibody Catenin beta 1 antibody Catenin beta-1 antibody CATNB antibody CHBCAT antibody CTNB1_HUMAN antibody CTNNB antibody CTNNB1 antibody DKFZp686D02253 antibody FLJ25606 antibody FLJ37923 antibody OTTHUMP00000162082 antibody OTTHUMP00000165222 antibody OTTHUMP00000165223 antibody OTTHUMP00000209288 antibody OTTHUMP00000209289 antibody
• Abbreviation: Catenin beta-1 Beta-catenin CTNNB1 CTNNB
• CAS Number: 9000-83-3
• Swiss Prot: P35222 Human, Q02248 Mouse, Q9WU82 Rat
• Cellular Locus: Cytoplasm, Nucleus, Cell membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser552 of Human Beta catenin.
• Isotype: IgG
• Clone: PSH08-72
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC
• Positive Control: HeLa cell lysate, HeLa treated with 10μM MG-132 for 6 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, C6 cell lysate, mouse colon tissue, C6 cells treated with 25μM MG-132 for 4 hours, human colon tissue, mouse liver tissue, rat colon tissue, NIH/3T3, C6.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 85 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes