Recombinant SARS-CoV-2 (SARS-CoV-2) S protein RBD (C-mFc-6His tag)

Recombinant SARS-CoV-2 (SARS-CoV-2) S protein RBD (C-mFc-6His tag)

• Background: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
• Abbreviation: Spike
• Synonyms: COVID-19, S glycoprotein RBD, S protein RBD, Spike glycoprotein Receptor-binding domain, Spike protein RBD
• CAS Number: 9000-83-3
• UniProt: P0DTC2
• Accession Number: QHD43416.1
• Host: HEK293 Cells
• Origin Species: SARS-CoV-2
• Tag: C-mFc-His
• Sequence: Arg319-Phe541
• Applications: ELISA
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng/ml. 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein（PKSR030492） in a linear range of 0.488-49.83 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.