Recombinant SARS-CoV-2 NSP13 protein

Recombinant SARS-CoV-2 NSP13 protein

• Background: NSP13 is a 67 kDa protein that belongs to the helicase superfamily 1B, it utilizes the energy of nucleotide triphosphate hydrolysis to catalyze the unwinding of double-stranded DNA or RNA in a 5′ to 3′ direction. Although NSP13 is believed to act on RNA in vivo enzymatic characterization shows a significantly more robust activity on DNA in in vitro assays with relatively weak non processive helicase activity when compared to other superfamily 1B enzymes. NSP13 has been shown to interact with the viral RNA-dependent RNA polymerase NSP1210,11, and acts in concert with the replication-transcription complex (NSP7/NSP8/NSP12). This interaction has been found to significantly stimulate the helicase activity of NSP13 possibly by means of mechano-regulation. In addition to its helicase activity, NSP13 also possesses RNA 5′ triphosphatase activity within the same active site, suggesting a further essential role for NSP13 in the formation of the viral 5′ mRNA cap.
• Synonyms: Helicase, SARS-CoV 2 nsp13
• CAS Number: 9000-83-3
• UniProt: P0DTC2
• Accession Number: QHD43415.1
• Host: E. coli
• Origin Species: SARS-CoV-2
• Tag: N-His
• Sequence: Ala5325-Gln5925
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.