Recombinant SARS-CoV-2 Papain-Like Protease Protein

Recombinant SARS-CoV-2 Papain-Like Protease Protein

• Background: Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
• Abbreviation: Papain-Like Protease
• Synonyms: PL-PRO, PLpro, Papain-like Protease, Replicase polyprotein 1a, pp1a
• CAS Number: 9000-83-3
• UniProt: P0DTC2
• Accession Number: QHD43415.1
• Host: E. coli
• Origin Species: SARS-CoV-2
• Tag: None
• Sequence: Glu1564-Lys1878
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Not Applicable
• Molecular Weight: 34 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
• Storage Conditions: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.