Recombinant SARS-CoV-2 Papain-Like Protease Protein

• Background:

  Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro) . These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.

• Abbreviation:

  Papain-Like Protease

• Synonyms:

  PL-PRO; PLpro; Papain-like Protease; Replicase polyprotein 1a; pp1a

• UniProt:

  P0DTC2

• Accession Number:

  QHD43415.1

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Glu1564-Lys1878

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  35.8 kDa

• Observed Molecular Weight:

  34 kDa

• Formulation:

  Supplied as a 0.2 µM filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.

• Citation 01:

  https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.2c02074

• Species:

  SARS-CoV-2