Recombinant Rat IL-6/Interleukin-6 Protein

Recombinant Rat IL-6/Interleukin-6 Protein

• Background: Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
• Abbreviation: IL-6/Interleukin-6
• Synonyms: IL6
• CAS Number: 9000-83-3
• UniProt: A6KJN3
• Accession Number: NP_036721.1
• Host: E. coli
• Origin Species: Rat
• Tag: None
• Sequence: Phe25-Thr211
• Applications: Cell culture
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 22 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.