Recombinant Human IL-6/Interleukin-6 Protein

Recombinant Human IL-6/Interleukin-6 Protein

• Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
• Abbreviation: IL-6;Interleukin-6
• Synonyms: IFNB, Interleukin HP, Interferon Beta, B-Cell Stimulatory Factor, IFN-Beta, B-cell hybridoma growth factor, B-Cell Stimulatory Factor 2, BSF2, BSF-2, CDF, CTL Differentiation Factor, HGF, HSF, Hybridoma Growth Factor, IFN-Beta-2, IL-6, Interferon Beta-2, Interleukin 6, Interleukin HP-1, Interleukin-6, IL6, IFNB2, B cell differentiation factor, B cell stimulatory factor 2, BSF 2, Hepatocyte stimulatory factor, Hybridoma growth factor Interferon beta-2, IL 6, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1
• CAS Number: 9000-83-3
• UniProt: P05231
• Accession Number: P05231
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Val30-Met212
• Applications: Cell culture
• Field of Research: Immunology,Neuroscience,Cancer,Metabolism
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: 1. Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately >5 x 10⁸ IU/mg.
  2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <4.4 ng/mL.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 22 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.