Recombinant Streptomyces hygroscopicus Bar Protein

Recombinant Streptomyces hygroscopicus Bar Protein

• Background: Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
• Abbreviation: Bar
• Synonyms: PPT N-acetyltransferase, Phosphinothricin N-acetyltransferase, Phosphinothricin-resistance protein, bar
• CAS Number: 9000-83-3
• UniProt: P16426
• Accession Number: P16426
• Host: E. coli
• Origin Species: Streptomyces hygroscopicus
• Tag: None
• Sequence: Met1-Ile183
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 18-20 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.