Recombinant Cynomolgus 5'-Nucleotidase/CD73/NT5E Protein (His Tag)

• Background:

  CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

• Abbreviation:

  CD73; NT5E

• Synonyms:

  5'-NT;5'-Nucleotidase; CD73; Ecto-5'-Nucleotidase; NT5; NT5E; NTE

• UniProt:

  G7P3J1

• Accession Number:

  G7P3J1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Trp27-Lys547

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  58.8 kDa

• Observed Molecular Weight:

  58-80 kDa

• Formulation:

  2

• Species:

  Cynomolgus macaques