Recombinant Human 5'-Nucleotidase/5'-NT/CD73 (AAH65937.1, C-Fc)

• Background:

  CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA) . The recombinant CD73 lacking GPI anchor is secreted as a monomer.

• Abbreviation:

  5'-Nucleotidase, 5'-NT, CD73

• Synonyms:

  5'-NT;5'-Nucleotidase; CD73; Ecto-5'-Nucleotidase; NT5; NT5E; NTE

• Accession Number:

  AAH65937.1

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Trp27-Lys547

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  84.6 kDa

• Observed Molecular Weight:

  85 - 100 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl₂, 20% Glycerol, pH 7.5.

• Species:

  Human