Recombinant Mouse Alpha-1-antitrypsin 1-1/Serpin A1e (C-6His)

• Background:

  Alpha-1-antitrypsin 1-5 (SERPIN A1) is a secreted protein and belongs to the serpin family. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. Mouse SERPIN A1 is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema orliver disease. Several transcript variants encoding the same protein have been found for this gene.

• Abbreviation:

  Serpin A1e

• Synonyms:

  Alpha-1 protease inhibitor 5; Alpha-1-antitrypsin 1-5; Dom5; Serine protease inhibitor 1-5; Serine protease inhibitor A1e; Serpin A1e; Serpina1e; Spi1-5

• UniProt:

  Q00898

• Accession Number:

  Q00898

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Glu25-Lys413

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  44.5 kDa

• Observed Molecular Weight:

  47 - 68 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

• Species:

  Mouse