Recombinant Mouse Interleukin-13/IL-13 Protein

Recombinant Mouse Interleukin-13/IL-13 Protein

• Background: Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.
• Abbreviation: Interleukin-13;IL-13
• Synonyms: BHR, Interleukin, T-Cell Activation Protein P, ALRH, BHR1, T-Cell Activation Protein P600, Interleukin-13, IL13, NC30, P600, Il-13
• CAS Number: 9000-83-3
• UniProt: P20109
• Accession Number: P20109
• Host: E. coli
• Origin Species: Mouse
• Tag: C-His
• Sequence: Pro22-Phe131
• Applications: Cell culture
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <4 ng/mL.The specific activity of recombinant mouse IL-13 is > 2.5 x 10⁵ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 11 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.