Recombinant Mouse CXCL2/MIP-2 Protein

• Background:

  C-X-C motif chemokine 2 (CXCL2, MIP-2) belongs to the intercrine alpha (chemokine CxC) family. It was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The expression of mouse MIP-2 is stimulated by endotoxin. The mouse MIP-2 shares approximately 63% aa sequence identity with murine KC, another mouse alpha chemokine, which is induced by PDGF. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

• Abbreviation:

  CXCL2; MIP-2

• Synonyms:

  C-X-C motif chemokine 2; GRO beta; GRO2; GROB; Gro-beta; Growth-regulated protein beta; MGSA-b; MGSA-beta; MIP-2; MIP2-alpha; MIP2A; Macrophage Inflammatory Protein-2-alpha; SCYB2.; chemokine ligand 2; cxcl2; melanoma growth stimulatory activity beta

• UniProt:

  P10889

• Accession Number:

  P10889

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Ala28-Asn100

• Applications:

  Cell culture

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  8.7 kDa

• Observed Molecular Weight:

  11 kDa

• Species:

  Mouse