Recombinant Mouse CD16-2/FCGR4 Protein (His&AVI Tag)

• Background:

  Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc γ receptors (FcR), and they are Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16) . Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc γ RIII (CD16) group.

• Synonyms:

  4833442P21Rik; CD16-2; FcgR4; FcgRIV; FcgammaRIV; Fcgr3a; Fcrl3; Low Affinity Immunoglobulin Gamma Fc Region Receptor IV

• UniProt:

  A0A0B4J1G0

• Accession Number:

  NP_653142.2

• Expression System:

  HEK293 Cells

• Tag:

  C-His-Avi

• Sequence:

  Met 1-Gln 203

• Applications:

  ELISA

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized mouse CD16-2-AVI-His at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) . The EC50 of human IgG1 (Fc) is 0.34-0.78 μg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  24.2 kDa

• Observed Molecular Weight:

  25-35 kDa

• Species:

  Mouse