Recombinant Mouse Activin RIIA/ACVR2A Protein (His Tag)

• Background:

  ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers) . Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.

• Synonyms:

  ActrIIa; Acvr2; TactrII

• UniProt:

  P27038

• Accession Number:

  NP_031422.3

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met 1-Pro 134

• Applications:

  Cell culture

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  14.8 kDa

• Observed Molecular Weight:

  35 kDa

• Species:

  Mouse