Recombinant Human CD59 (C-6His)

• Background:

  CD59, also known as membrane attack complex inhibition factor (MACIF), Protectin, 1F5 antigen, HRF-20 and MIRL, is an approximately 20 kDa GPI anchored glycoprotein that is an important regulator of the complement system in blood. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

• Abbreviation:

  CD59

• Synonyms:

  16.3A5;1F5;1F5 antigen;20 kDa homologous restriction factor; CD59; CD59 antigen; CD59 glycoprotein; EL32; FLJ38134; FLJ92039; G344; HRF-20; HRF20; MAC-IP; MACIF; MIC11; MIC11MSK21; MIN1; MIN2; MIN3; MIRL; p18-20

• UniProt:

  P13987

• Accession Number:

  P13987

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Leu26-Asn102

• Field of Research:

  Signal Transduction; Cardiovascular; immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  9.8 kDa

• Observed Molecular Weight:

  15 - 20 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human