Recombinant Human Signal Transducer CD24/CD24 (C-Fc-Avi) Biotinylated

• Background:

  Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.

• Abbreviation:

  CD24

• Synonyms:

  CD24; CD24A; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

• UniProt:

  P25063

• Accession Number:

  P25063

• Expression System:

  HEK293 Cells

• Conjugation:

  Biotinylated

• Tag:

  C-Fc-Avi

• Sequence:

  Ser27-Gly59

• Applications:

  ELISA

• Field of Research:

  Neuroscience; Cancer; immunology; metabolism; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized Anti-Human CD24 mAb at 2μg/ml (100 μl/well) can bind Biotinylated Human CD24-Fc-Avi. The ED50 of Biotinylated Human CD24-Fc-Avi is 1. 83 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  31.6 kDa

• Observed Molecular Weight:

  40 - 55 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human