Recombinant Human Tumor-associated Calcium Signal Transducer 2/TROP-2 (248AA, C-6His)

• Background:

  Tumor associated calcium signal transducer 2 (TACSTD2, TROP-2) is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. Human and mouse TROP-2 share 87% amino acid (aa) similarity. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

• Abbreviation:

  TROP-2

• Synonyms:

  Cell surface glycoprotein Trop-2; Membrane component chromosome 1 surface marker 1; TACSTD2; TROP2; Tumor-associated calcium signal transducer 2

• UniProt:

  P09758

• Accession Number:

  P09758

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  His27-Thr274

• Applications:

  BLI

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Anti-Human TROP-2 mAb-Fc on Protein A Biosensor, can bind Human TROP-2-His with an affinity constant of 0.21 nM as determined in BLI assay.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  28.9 kDa

• Observed Molecular Weight:

  40 - 50 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human