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Recombinant Human Wnt3a/Wnt-3a

CAT: 0763-PKSH033972-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0763-PKSH033972-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity) . Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) .
Abbreviation
Wnt3a; Wnt-3a
Synonyms
MGC119418; MGC119419; MGC119420; Wnt-3a; Wnt3a; member 3A; protein Wnt-3a; wingless-type MMTV integration site family
Expression System
HEK293 Cells
Tag
None
Applications
Cell culture
Endotoxin
< 0.01 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
58.5 kDa
Observed Molecular Weight
66-73 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.
Species
Human
Product Datasheet
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