Recombinant Human Interleukin-13/IL-13 (C-mFc)

• Background:

  Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.

• Abbreviation:

  Interleukin-13, IL-13

• Synonyms:

  BHR; Interleukin; T-Cell Activation Protein P; ALRH; BHR1; T-Cell Activation Protein P600; Interleukin-13; IL13; NC30; P600; Il-13

• UniProt:

  P35225

• Accession Number:

  AAK53823

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc

• Sequence:

  Gly21--Asn132

• Applications:

  Cell culture

• Field of Research:

  Immunology, Cancer, Kits, Lysates, Other, Cardiovascular

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells The ED₅₀ for this effect is 300 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  38.7 kDa

• Observed Molecular Weight:

  38 - 60 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human