Recombinant Human Interleukin-23/IL-23 (C-6His)

• Background:

  Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.

• Abbreviation:

  Interleukin-23; IL-23

• Synonyms:

  CLMF p40; IL-12 subunit p40; IL-23p19; NKSF2; SGRF

• UniProt:

  Q9NPF7; P29460

• Accession Number:

  Q9NPF7&P29460

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Arg20-Pro189&Ile23-Ser328

• Applications:

  Cell culture

• Field of Research:

  Microbiology; Cancer; immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  19.7&34.8 kDa

• Observed Molecular Weight:

  19-21&40-60 kDa

• Species:

  Human