Recombinant Human Interleukin-36 alpha/IL-36 alpha

• Background:

  Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

• Abbreviation:

  IL-36A; IL-36 alpha

• Synonyms:

  FIL; IL1F; Interleukin-1 Family Member; IL-1F; IL36A; FIL1; FIL1 (EPSILON) ; FIL1E; IL-1F6; IL1 (EPSILON) ; IL1F6; FIL1 Epsilon; IL-1 Epsilon; Interleukin-1 Epsilon; Interleukin-1 Family Member 6; Interleukin-36 Alpha; IL1E; IL 1 epsilon; IL 1F6; IL 1H1; IL1RP2; Interleukin 1 epsilon; Interleukin 1 family member 6; Interleukin 1 family member 6 (epsilon) ; Interleukin 36 alpha; MGC129552; MGC129553; MGC151479; MGC151481; OTTMUSP00000012798; RP23-176J12.4

• UniProt:

  Q9UHA7

• Accession Number:

  Q9UHA7

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Lys6-Phe158

• Applications:

  Cell culture

• Field of Research:

  Immunology

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.1 kDa

• Observed Molecular Weight:

  17 kDa

• Species:

  Human