Recombinant Human DPP4/CD26 Protein

Recombinant Human DPP4/CD26 Protein

• Background: Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.
• Synonyms: ADABP Protein, ADCP2 Protein, CD26 Protein, DPPIV Protein, Human, TP103 Protein
• CAS Number: 9000-83-3
• UniProt: P27487
• Accession Number: NP_001926.2
• Host: HEK293 Cells
• Origin Species: Human
• Tag: None
• Sequence: Asn29-Pro766
• Applications: BLI
• Field of Research: Cell biology;Cancer;immunology;metabolism;Stem cells
• Purity: > 70 % as determined by reducing SDS-PAGE.
• Bioactivity: 1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2, 500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human DPP4/CD26 Protein(Active)(Cat: PKSH033811) bound to Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) (Cat: PKSV030236) was 33 nM. 3. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human DPP4/CD26 Protein(Active)(Cat: PKSH033811) bound to Recombinant HCoV-HKU1 (Isolate N1) S1 Protein (His Tag) (Cat: PKSV030109) was 12 nM.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 95 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.